PTEN and hepatocellular carcinoma: Of note, the concomitant deletion of both Tsc1 and Pten in the liver gave rise to the rapid induction of HCC, usually around the age of 14 weeks, showing the simultaneous deletion of the two major negative regulators of PI3K/AKT/mTOR signaling strongly enhanced the signaling pathway and carcinogenesis in the liver [63].