The0.8 MPa exposure enhanced perfusion substantially, whereas the2.4 MPa reduced blood perfusion. There was a significant increase in CD8+ T cells for the0.8 MPa exposure compared to the untreated tumours and US+CA treatment at2.4 MPa. Moreover, US+CA+aPD-L1 had significantly better therapeutic effect and more CD8+ T cells than US+CA and aPD-L1 only. Additionally, the US+CA+aPD-L1 boosted IFN-γ and granzyme B secretion. This evidence concerns the gene CD8A and neoplasm.