In this study, metabolites derived from F. nucleatum exhibited no inhibitory effects on the cell growth of the normal colon and CRC cell lines, except for a notable and exclusive up-regulation of expression of TLR4 and Myc and genes encoding pro-inflammatory cytokines, including IL1B, IL6, and IL8 in Caco-2 cells, when F. nucleatum cultured in normal BHI media. This evidence concerns the gene IL1B and colorectal carcinoma.