INS and metabolic dysfunction-associated steatotic liver disease: Proposals to treat non-alcoholic fatty liver disease (NAFLD) NRK1 by targeting HS are based on the discovery that in aged mice or in mice treated with HFD, NRK1 overexpressed by adenovirus-mediated gene transduction regulates lipid metabolism (mainly TG level) and NAD+ biosynthesis in the liver, insulin sensitivity, and glucose tolerance [98].