As shown in Figure 3B, two-way ANOVA showed that genotype (F(1,20) = 6.206, p = 0.0216) and aerobic exercise (F(1,20) = 19.72, p = 0.0003), but not the interaction (F(1,20) = 3.929, p = 0.0614), exerted main effects on the activation of AKT in the brains of the mice, and post-hoc analyses showed that the p-AKT/AKT ratio was significantly decreased in the ADC group of mice compared with the WTC group mice (p < 0.01); moreover, aerobic exercise significantly increased the p-AKT/AKT ratio in the brains of mice in the ADE group compared with mice in the ADC group (p < 0.001). Here, AKT1 is linked to acute disseminated encephalomyelitis.