To verify whether cleft palate induced by plerixafor in fetal mice had reduced Cxcr4 gene expression and was associated with impaired migratory function as in vitro, we then performed protein extraction from palatal tissues and examined the expression of CXCR4, additionally with RAC1, CDC42, and RHOA expressions, which are core markers in cell migration in different groups. This evidence concerns the gene CDC42 and cleft palate.