The role of LMNB1 in nuclear integrity has been previously validated by an in vitro cell system based on the human osteosarcoma cell line U2OS, which showed a 60% reduced expression level of LMNB1 (U2OS GFP-NLS shLmnB1) and a significantly higher tendency of spontaneous rupture of the nuclear envelope [80,81,82]. Here, LMNB1 is linked to osteosarcoma.