We observed an interaction between the two factors analyzed in Gls mRNA expression [F (1,28) = 21.23; p < 0.001], with 3×Tg-AD female astrocytes showing a lower level of Gls than WT female and 3×Tg-AD male groups (Tukey’s multiple comparisons test; **/###p < 0.01/0.001; Figure 6E). The gene discussed is GLS; the disease is Alzheimer disease.