Two-way ANOVA indicated a sex effect on Tnfa mRNA levels [F (1,28) = 6.865; p < 0.05], with decreased Tnfa mRNA levels in hippocampal astrocytes of 3×Tg-AD female offspring compared to 3×Tg-AD male astrocytes (uncorrected Fisher’s LSD post hoc test; * p < 0.05; Figure 1C). This evidence concerns the gene TNF and Alzheimer disease.