Studies on obesity have shown that NK6 homeobox 1 (Nkx6.1), which binds NOTCH1 at a 139 bp enhancer sequence (known as the CR2 fragment) in the second intron and positively regulates its expression [81], is upregulated by lncRNA regulator of insulin transcription ROIT [82]. Here, NKX6-1 is linked to obesity due to melanocortin 4 receptor deficiency.