Significantly higher levels of 5hmC of gene B4GALT5 were also observed in the gDNA of colon cancer tissue samples versus adjacent normal samples, where the estimated value of colon cancer tissue was 1.80 RPKM; 0.32 RPKM higher than in the gDNA of normal adjacent tissue. The gene discussed is B4GALT5; the disease is malignant colon neoplasm.