GLS1 silencing significantly increased the percentage of dead cells from 7.6% of wt CAL-62 to the 13.5% of GLS1- after 24 h and from 8.5% of wt CAL-62 to 18.7 of GLS1- after 48 h, indicating a deleterious effect of GLS1 reduction in this cancer cell line (see Figure 1G,J and Figure 1H,K). The gene discussed is GLS; the disease is cancer.