In murine IRI-AKI, the upregulated expression of RIPK3 and MLKL in renal proximal tubular cells induced necroptosis and contributed to NLRP3 inflammasome activation, and Ripk3 or Mlkl deficiency reduced tubular cell necroptosis, macrophage infiltration, and NLRP3 inflammasome activation [80]. Here, MLKL is linked to acute kidney injury.