Melatonin treatment significantly counteracted the obesity-induced changes for MyH7 (0.85 ± 0.05 AU), MyH2 (1.10 ± 0.07 AU), and MyH4 (0.21 ± 0.05 AU) expression in the ZDF group while conversely had no effects for MyH1 expression (0.33 ± 0.05 AU), as compared to the respective control group (p < 0.01, p < 0.01, and p < 0.01, respectively; Figure 6). This evidence concerns the gene MYH1 and obesity due to melanocortin 4 receptor deficiency.