Therefore, here, we report the protective antioxidant and anti-inflammatory effects against hyperglycemia-induced oxidative stress by AuNPs and L. barbarum extract in vitro on endothelial cells with respect to oxidative-stress-induced lipid peroxidation antioxidant defense mechanisms and the synthesis of proinflammatory cytokines interleukin 1 α (IL1α) and interleukin 1β (IL1β). Here, IL1B is linked to Hyperglycemia.