To further assess the role of complete or partial Ppp2r5d loss on the initiation and/or progression of HCC in a chronic liver injury model, Ppp2r5d +/+ (WT), Ppp2r5d +/− (HE), and Ppp2r5d −/− (HO) male mice were injected with 20 mg/kg DEN at 2 weeks of age. The gene discussed is PPP2R5D; the disease is hereditary elliptocytosis.