The renal fibrosis amelioration was confirmed by the reduction in mRNA levels of Acta2, Collagen I, Collagen III, Mmp2, and Mmp9 (Figure 5E) and the protein levels of ACTA2, fibronectin, and Collagen I (Figure 5F) in UI30/2wk Pfkfb3f/f/PostnMCM kidneys compared to PostnMCM kidneys. The gene discussed is ACTA2; the disease is renal fibrosis.