AFDN and type 2 diabetes mellitus: The immunohistochemistry results showed changes consistent with the quantitative proteomics results, as depicted in Figs. 6A and B. Pixel analysis showed that the expression levels of MLLT4 and CTNND1 were significantly higher in NG samples than in PTDM samples, and MLTT4, CTNND1 and LHPP expression levels were lower in T2DM samples than in NG samples.