Finally, using four different published patient datasets, we found that genes associated with MLL-AF4-unique enhancers were significantly overexpressed in MLLr patient samples compared to other ALL subtypes or normal preB cells (Supplementary Fig. 2g; ECOG E299348 84 genes up vs 36 down, p = 1.4 × 10−5; COG P990649 114 genes up vs 16 down, p < 1 × 10−10; St. Here, AFF1 is linked to acute lymphoblastic leukemia.