To clarify whether the rescuing effects of CISD KD and KO are due to reduced IP3R activity, we observed PD-related phenotypes in PINK1 or Parkin null flies co-expressing CISD RNAi and exogenous Drosophila IP3R. While CISD KD and KO in PINK1 and Parkin null flies completely rescued PD-related phenotypes (Fig. 5a–f and Supplementary Figs. 9a–h, 10a–f, and 11a, b), simultaneous expression of IP3R resulted in recurrence of PD-related phenotypes, indicating that the rescuing effects of CISD KD and KO were strongly diminished (Fig. 5a–f and Supplementary Figs. 9a–h and 11a, b). Here, ITPR1 is linked to Parkinson disease.