To analyze the effects of REG1α on the activity of Wnt/β-catenin signaling in CRC cells, TOP/FOPFLASH reporter assay was conducted, and the results showed that overexpression of REG1α markedly augmented the transcriptional activity of β-catenin, whereas silencing REG1α impaired Wnt/β-catenin pathway activity (Fig. 5A). This evidence concerns the gene REG1A and colorectal carcinoma.