In DMD studies, for example, ∼2.4-Mb whole dystrophin gene was deleted in mice (Kudoh et al., 2005), and our group also successfully introduced a 342-kb deletion at the dystrophin gene (exon 45–55) in iPSCs by Cre-loxP recombination (Kagita et al., 2021). This evidence concerns the gene DMD and Duchenne muscular dystrophy.