HLTF and Glucose intolerance: Fig 2D shows PCR validation of the genotype. Because rIPCre mice alone display glucose intolerance as early as 6 weeks of age [24, 25], it was imperative to include all the appropriate controls to show Hltf fl/fl as well as rIPCre Hltf +/+ mice have normal blood sugar at birth compared with Hltf +/+ control pups (Fig 2B). IC global and β Hltf KO mice share the same dramatic reduction in circulating levels of blood glucose and the same perinatal lethal phenotype indicating it is solely attributable to alterations in islet β cells (Fig 2B).