Our results showed that SFTSV infection was significantly decreased at 24h, 48h and 72h in cells with Filipin treatment compared with the wild-type cells, and more significantly, it was decreased in Filipin treated CLTC-KO cells (Fig 4E), while it was not significantly decreased in wild-type cells with EIPA treatment, as shown in S2 Fig. The gene discussed is CLTC; the disease is infection.