HEK293T cells were treated with a RIG-I inhibitor (Cyclo Phe-Pro, a Vibrio vulnificus quorum-sensing molecule, inhibits RIG-I polyubiquitination) and RIG-I agonists (KIN1408, an agonist of the RIG-I pathway) to determine whether IFN-I induces ISGs expression and affects virus infection levels by qRT-PCR. The gene discussed is RIGI; the disease is viral infectious disease.