And, time spent in the center zone was remarkably increased after Wnt3a (t(14) = 4.561, p = 0.0005) or Lip-1 (t(14) = 5.256, p ˂ 0.0001) treatment following TBI (Fig. 7D), indicating that Wnt3a or Lip-1 intervention both attenuated the anxiety level after TBI. Here, WNT3A is linked to Anxiety.