In the present study, four V. cholerae O1 classical biotype strains (O395, 569B, Cairo48, and Cairo50) and five El Tor biotype strains (N1696, T19479 (belonging to the Wave 1 El Tor biotype strain), B33, MG116025 (classified as a Wave 2 El Tor biotype strain), and IB5230 (a 2010 Haitian cholera outbreak strain that is grouped as a Wave 3 El Tor biotype strain)) and isogenic derivatives of each strain that contained variant toxT alleles, or had the toxT gene deleted (ΔtoxT), were analyzed for CT/TCP expression under laboratory culture conditions (Table 2). Here, SPINK1 is linked to vibrio infectious disease.