PCK1 and Hypoglycemia: Consistent with the observed relative fasting hypoglycemia with 5 mg/kg methomyl (Supplemental Figure S3), mRNA expression of phosphoenolpyruvate carboxykinase (Pck1), the rate limiting step in hepatic gluconeogenesis, was significantly increased 1.7-fold (p = 0.0006) (Figure 3C).