According to our data, VEGF/PDGFr immunofluorescence signal, although overexpressed in all cholesteatomas, showed significantly higher values in acquired cholesteatomas as compared to congenital ones (p < 0.001), particularly at the peri-matrix level, where the significantly higher mean OD were detected in the children’s population (p < 0.001). The gene discussed is VEGFA; the disease is cholesteatoma.