To explain the mechanisms of iron deficiency in patients with PDAC, we assessed the expression of the iron regulators HEP, FPN, and ZIP14 in PDAC tissue (n = 56) to determine whether they can be expressed locally by the tumor and influence the tumor’s iron homeostasis, as well as the mitochondrial mass to search for the link between iron regulators and mitochondrial metabolism within PDAC. This evidence concerns the gene SLC40A1 and Iron deficiency anemia.