To investigate the relationship between PGAM5 and depression, we first used CUMS‐treated mice, which displayed reduced central walking distance in the OFT (p < 0.05 for Control vs. CUMS; Figure 1A–C) and increased immobility time in the forced swim test (FST) (p < 0.01 for Control vs. CUMS; Figure 1D). This evidence concerns the gene PGAM5 and major depressive disorder.