There were 97 cases of RMS including 39 of ARMS, 43 of ERMS, 14 of PRMS, and one of SRMS, in which 93 samples were tested for PAX3–FOXO1 fusion transcripts by one-step RT-PCR, and other four samples could not be tested because of poor RNA quality. Here, PAX3 is linked to alveolar rhabdomyosarcoma.