Ultimately, ten hub genes (CD74, COL1A1, TXNRD1, CCND1, COL5A1, SERPINH1, BCL6, ITGA10, FOS, and JUNB) were obtained and the validation of expression levels was performed to elucidate the molecular mechanism of AS and AI pathological processes. This evidence concerns the gene COL5A1 and aortic stenosis.