Finally, the mRNA expression values of ten hub genes (CD74, COL1A1, TXNRD1, CCND1, COL5A1, SERPINH1, BCL6, ITGA10, FOS, and JUNB) were validated by analyzing results of high throughput RNA sequencing from AS and AI aortic valve samples and by examining the mRNA expression levels of human AS and AI aortic valve tissues. This evidence concerns the gene COL1A1 and aortic valve stenosis.