Finally, the mRNA expression values of ten hub genes (CD74, COL1A1, TXNRD1, CCND1, COL5A1, SERPINH1, BCL6, ITGA10, FOS, and JUNB) were validated by analyzing results of high throughput RNA sequencing from AS and AI aortic valve samples and by examining the mRNA expression levels of human AS and AI aortic valve tissues. Here, CCND1 is linked to aortic valve stenosis.