To further clarify the intrinsic molecular mechanism of FSH and S1P co-intervention to protect the survival of follicles in transplanted ovaries, we treated human granulosa-like tumor cell line (KGN) with ischemia and hypoxia in vitro to simulate the state of ischemia and hypoxia in early transplanted ovaries and evaluated the protective effect of FSH and S1P co-intervention on ischemia-hypoxia-induced KGN cells. This evidence concerns the gene MBTPS1 and neoplasm.