SPEN and cancer: Moreover, TMB was significantly different among SPEN missense mutant cancer (18.0, 5.3–45.7), and cancer with multiple mutations (79.0, 42.1–329.0; Fig. 6B), and TMB stratified by SPEN mutation status in different cancer type were shown in Fig. 6C. A remarkable relationship between the frequencies of SPEN mutation and median TMB was observed in different cancer types (R = 0.845; P < 0.001; Fig. 6D).