Given the important role of SPEN mutation in pan-cancer, the correlation between SPEN mutation and tumor-infiltrating lymphocytes, immunoinhibitors, immunostimulators, major histocompatibility complex molecules (MHC), chemokines, and chemokine receptors was investigated, in STAD (n = 35), BRCA (n = 32), SKCM (n = 30), COAD (n = 31), BLCA (n = 27), HNSC (n = 27) and LUAD (n = 20), seven tumors with over 20 SPEN mutant cases in TCGA cohort (Fig. 5A-F). The gene discussed is HLA-C; the disease is cancer.