To investigate MKRN1's influence on CRC development in vivo, we constructed Apc [MU +] MKRN1 CKO mice (Apc [MU/ +], Mkrn1 [+ / + , pVillin-Cre], Apc [MU/ +], and Mkrn1 [flox/flox, pVillin-Cre]). The gene discussed is APC; the disease is colorectal carcinoma.