Subsequently, to test the need for a properly functioning biological clock in T-ALL cell lines as well as to assess whether leukemia T-cells are dependent on BMAL1 and CLOCK gene expression, SUP-T1 and RPMI-8402 cell lines were transduced with scramble control or shBMAL1 or shCLOCK constructs for knocking-down BMAL1 and CLOCK gene, respectively (Fig. S3). This evidence concerns the gene BMAL1 and acute lymphoblastic leukemia.