To validate the in vivo imaging results, we used fluorophore-conjugated anti-mouse CD3 and CD8 antibodies, RBD–tetramer and OVA–tetramer to perform ex vivo flow cytometry analysis of cells extracted from tumours (Fig. 4f and Supplementary Figs. 18, 19, 22 and 23 used protein kinase inhibitor (PKI) dasatinib for optimized tetramer staining38; Supplementary Figs. 20, 21, 24 and 25 show cells without PKI). This evidence concerns the gene CD8A and neoplasm.