Some of these genes have been previously reported to show altered expression in both NAFL and NASH stages, including epigenetic genes such as DNMT1, SIRT1, and ZBTB33, and epitranscriptomic genes like IGFBP1. However, for most of these genes, their differential expression in NAFL or NASH had not been previously described. Here, SIRT1 is linked to metabolic dysfunction-associated steatohepatitis.