Interestingly, several previous in vivo and in vitro studies have demonstrated that various antagonists, including nuciferine79, hederacoside-C80, chlorogenic acid81, polydatin82, curcumin83, and indirubin84, attenuate the phosphorylation of MAPKs and NF-κB through suppression of TLR2 and TLR4, thereby preventing hyperinflammation and immunopathology induced by LPS and S. aureus infection in mastitis. This evidence concerns the gene TLR4 and mastitis.