In the tumor of mice treated with both, radiation and CTLA4 inhibition, CD4TH1 and CD8EX are present in similar proportion but in addition CD4 regulatory (CD4TREG) cells are reduced while CD8 effector memory (CD8EM), early activation (CD8EA) and precursor exhausted (CD8PEX) T cells are expanded compared to control and monotherapy-treated mice. The gene discussed is CD8A; the disease is neoplasm.