In contrast, in the ASL-modulated tumors, Dox triggered a more notably elevated fluorescence intensity of CRT and HMGB1 release (Fig. 6g), likely due to both the adjuvant properties of Ausome and a heat-facilitated increase in tumor Dox perfusion (Fig. 6g, Supplementary Fig. 22). This evidence concerns the gene HMGB1 and neoplasm.