Subsequent qPCR with reverse transcription (qRT–PCR) examination validated the significant suppression for Vhl expression, while no significant alteration for Hif1α level was observed in eWAT and iWAT from 231/Ctrl mice when compared to tumour free mice or 231/Rab27a KD mice (Fig. 2b). This evidence concerns the gene HIF1A and neoplasm.