To finally determine to what degree BC tumours dysregulate adipose tissue through miR-204/VHL axis, we constructed adeno-associated virus 9 (AAV9) expressing mouse Vhl cDNA and injected the AAV-VHL (TB AAV-VHL) into adipose tissue following BC tumour implantation, with AAV-GFP (TB AAV-GFP) served as a control. Here, VHL is linked to breast cancer.