As expected from previous publications, WT (i.e., Ccne1f/f) mice developed pronounced liver fibrosis with excessive hepatic collagen deposition upon combined DEN/CCl4 treatment (Fig. 2B, C), which was accompanied by substantial induction of Col1a1 gene expression and upregulation of Acta2 (Fig. 2D, E). Here, ACTA2 is linked to Hepatic fibrosis.