PROM1 and hepatocellular carcinoma: We next examined if the absence of Ccne1 in HSCs may affect the expression of genes encoding albumin (Alb), α-fetoprotein (Afp), and CD133 (Prom1), which are established markers for hepatocytes (Alb), de-differentiated hepatoma cells (Afp) or liver cancer stem cells (Prom1) [14–16].