Moreover, the analysis of mucosal barrier protein expression in rectal tissue samples revealed that CGA downregulated the MLCK/p‐MLC signaling pathway proteins (Figure 2K), stabilized the expression of TJ proteins (ZO‐1 and occludin) (Figure 2L, N and O), and reduced serum FITC‐dextran levels, reversing the increased intestinal permeability in PI‐IBS rats (Figure 2M). The gene discussed is OCLN; the disease is irritable bowel syndrome.