Collectively, these results indicate that the canonical NLRP3 and NAIP/NLRC4 inflammasomes, as well as broadly ASC-dependent inflammasomes, are not activated during Δ6 Yptb infection of Caco-2 cells, and that GSDMD cleavage and activation occur downstream of caspase-4 and are required for cell death and cytokine release. This evidence concerns the gene NLRP3 and infection.