This acetylcholine-induced response was also improved in SLE after incubation with the pan-NOX inhibitor VAS2870 or the Rho kinase inhibitor Y27632 (Figure 5a), suggesting that the impairment in acetylcholine-induced relaxation is mediated, at least in part, by NADPH oxidase and Rho kinase activation. Here, FMO5 is linked to systemic lupus erythematosus.