Incubation for 30 min with the pan-NOX inhibitor VAS2870 or the Rho kinase inhibitor Y27632 abolished differences between groups in relaxation to acetylcholine, showing the involvement of NADPH oxidase and Rho kinase in this impaired relaxant response induced by SLE microbiota (Figure 7a). The gene discussed is FMO5; the disease is systemic lupus erythematosus.