Insulin concentrations in late pregnancy were positively associated with DNAm changes at birth of the RNF214 and PCSK7 genes;Maternal indicators of insulin resistance and β-cell function in early pregnancy were associated with lower methylation near the SYN3 and JARID2 genes;maternal insulin sensitivity was associated with higher methylation on the POLR2C gene;no intervention effect on newborn DNAm,. Here, RNF214 is linked to Insulin resistance.