In this study, we successfully established an NAFLD model by feeding mice with an HFD diet for 12 weeks, as evidenced by hepatic lipid deposition (increased hepatic TG and hepatic lipid droplets), and hepatic impairment (elevated serum ALT and AST levels) in HFD fed mice (Amernia et al., 2021). The gene discussed is GPT; the disease is metabolic dysfunction-associated steatotic liver disease.