A detailed inspection of the RSPO2 promoter region revealed its consistent hypermethylation in CRC samples and CRC cell lines as compared to control tissue (Fig. 5A and 5B) in addition to the significant DMPs identified in the DNA methylation analysis (cg04050867, cg20061155, cg14070647 and cg09970569, Additional file 12: Fig. S3A and S3B), while an opposite trend was observed in the context of CpGs located at the intragenic region (Fig. 5A and 5B). This evidence concerns the gene RSPO2 and colorectal carcinoma.