To better investigate the interaction between BLACAT3 and YBX3, we detected the protein level of YBX3 in T24 and 5637 and found no significant difference in YBX3 protein levels in both BLCa cells with BLACAT3 knockdown or overexpression, suggesting that BLACAT3 had no effect on YBX3 expression (Fig. 7E, H). This evidence concerns the gene YBX3 and bladder transitional cell carcinoma.